Part:BBa_K121017:Design

promoterless gfp

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 27
  Illegal BamHI site found at 15
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 714
  

Design Notes

We deleted the promoter of BBa_I7100 and inserted newly designed prefix and suffix each of which has 2 restriction-enzyme-sites(prefix: SalI and BamHI,surfix: BglII and MulI) But we did not insert new promoter.

Source

BBa_I7100